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 Indian Rocks Beach, FL   33785

 (727) 642-0182

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"Helping People Learn Compliance and Science" SM

 

Introduction To High Performance

Liquid Chromatography (HPLC)

 

 

ABOUT THE COURSE

“Introduction to High Performance Liquid Chromatography (HPLC)” is a solid, one-day course focusing on the practical aspects of using HPLC in the modern analytical laboratory. The course materials are based on current best practices of the pharmaceutical industry and are tied to real world examples whenever possible. Emphasis is based on understanding chromatography from a molecular separation standpoint. The course is heavily oriented towards personnel who use or will use HPLC on a daily basis, and to providing them the skills and understanding to become more productive and confident in there work. This is an excellent starting point for personnel just beginning to use HPLC and a great re-introduction to personnel who have been using HPLC for some time.

WHO SHOULD ATTEND

All laboratory personnel who use HPLC in the execution of their job responsibilities will benefit from this course. It is also an excellent means to cross-train personnel QA personnel prior to their review of data generated by HPLC analyses.

COURSE OUTLINE

1. Introduction: Anatomy and History of Chromatographic Separations
2. Chromatography in the Modern Laboratory
     a. Types of Chromatography
     b. Modern Applications
     c. Definition of HPLC and Modes of Separation
     d. Equations
3. Components of a Modern HPLC System
     a. Hardware
     b. Columns and Stationary Phases
     c. Mobile Phases
     d. Data Acquisition and Processing
4. Chromatographic Methods Development
     a. End User Requirements Analysis
     b. Molecular Analysis and Mode Selection
     c. Column Selection
     d. Mobile Phase Selection
     e. Systematic Screening: Pilots Studies
     f. Robustness Testing
     g. Final Method Selection and Documentation
5. Qualitative Analyses
6. Quantitative Analyses
7. Overview of Methods Validation
8. Troubleshooting , Tools and Tricks
9. Review of Selected References
10. Where to Get Help
11. Questions and Answers

ADMINISTRATION

The course is one day in length. Cost is $525 per person, with a five-student minimum for on-site presentation. Payment can be made by personal check, company check, purchase order, or by MasterCard, Visa, or American Express. Registration can be made on-line or via telephone.

 

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