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Delphi Analytical Services, Inc. in Partnership with Alturas Analytics, Inc. Presents:

HPLC Methods Development

For LC/MS Analyses

ABOUT THE COURSE

“HPLC Methods Development for LC/MS Assays” is a solid, one-day, in-depth course focusing on the practical aspects of developing good HPLC methods to maximize the quality, efficiency, and effectiveness of your LC/MS analyses.

WHO SHOULD ATTEND

Experienced analytical chemists, who are involved in the development of HPLC methods and daily performance of LC/MS or LC/MS/MS analyses, will benefit greatly from this course.

ABOUT THE INSTRUCTOR

The primary instructor is DR. SHANE NEEDHAM, Ph.D. Dr. Needham is Laboratory Director of Alturas Analytics, Inc where he manages the development and validation of HPLC/MS/MS assays to support the pharmaceutical industry. He has more than 12 years of experience in analytical laboratories and has over 10 years of LC/MS and analytical lab experience in the drug discovery through drug development stage in the pharmaceutical industry. Shane previously worked for Pfizer Central Research in Groton, CT where he developed and validated LC/MS/MS methods for the trace analysis of drugs and metabolites in biological fluids. He also performed in vivo and in vitro structure elucidation studies of drugs and metabolites using HPLC/MS/MS. Shane’s Ph.D. work included the development of optimized stationary phases to improve the assay of pharmaceuticals by HPLC/MS. Shane has over 60 publications and external presentations in the area of LC/MS and teaches LC/MS courses on the national and international level. Shane is a member of the American Chemical Society, Sigma Xi and American Society for Mass Spectrometry and American Association of Pharmaceutical Scientists.

COST

The course is one day in length. Cost is $525 per person, with a ten-student minimum for on-site presentation. Payment can be made by personal check, company check, purchase order, or credit card. Discounts for larger on-site groups are offered.

HPLC METHODS DEVELOPMENT FOR LC/MS ASSAYS COURSE OUTLINE

1. Introduction
    a. Brief History of Coupling HPLC and Mass Spectrometry
2. Interfacing HPLC with Atmospheric Pressure Ionization
    a. Electrospray Ionization (ESI)
    b. Atmospheric Pressure Chemical Ionization (APCI)
    c. Optimum Operating Conditions
3. Developing HPLC Methods for MS Detection
    a. Define final intended use for method
    b. Obtain basic chemical information on analytes
    c. Obtain basic information on biological matrices to be analyzed
    d. HPLC Column Chemistry Choice
    e. HPLC Column Size Choice
    f. Buffer and Solvent Choice for LC/MS
    g. Choice of Mode of HPLC Separation
    h. Optimization of column temperature for LC/MS
    i. Column Switching Applications
4. HPLC/MS Methods: Experiments and Results
    a. Where to start for a typical HPLC/MS method
    b. What is adequate retention and peak shape?
    c. Desired Results and What to Expect
5. Typical Problems, Solutions and Troubleshooting HPLC for MS Detection
    d. Matrix Interferences and Ion-Suppression in LC/MS
    e. The Transfer and Improvement of HPLC Methods
    f. How to improve analysis time without sacrificing quality data
    g. How to improve chromatographic peak shape
    h. How to improve chromatographic retention
    i. Simultaneous HPLC/MS analysis of molecules with different properties
    j. LC/MS Survival Kit-Tips and Tricks for What you really need to know
6. Group Problem Solving-Case Studies in LC/MS Method Development
    a. Dissecting some case studies for specific problems encountered in the laboratory
    b. Method development schemes for specific classes of molecules
7. Questions and Answers

ENROLLMENT

Delphi Analytical Services, Inc. (DAS) offers this course at the customer site or at conveniently located facilities around the country. If you are interested in attending the course please contact:

 

Delphi Analytical Services, Inc.
P.O. Box 487
Indian Rocks Beach, FL 33785
727-642-0182
Fax 727-593-3595
info@delphianalytical.com
www.delphianalytical.com

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